liaF [2019-06-06 09:44:59]

inhibitor of LiaS kinase activity

Locus: BSU33100

Isoelectric point: 9.21

Molecular weight: 26.88 kDa

Protein length: 241 aa

Gene length: 726 bp

Function: control of LiaR activity

Product: negative effector of LiaS

Essential: no

Synonyms: yvqF

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Control of response regulators/ other
• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,396,114 → 3,396,839

The protein

Catalyzed reaction/ biological activity

• inhibitor of LiaS kinase activity, maintains LiaS in the phosphatase state in the absence of the stress signal PubMed

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: liaG-liaF-liaS-liaR
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • LiaR: activation, PubMed, in LiaR regulon

  Regulation

  • liaG: constitutive

• Genes: liaI-liaH-liaG-liaF-liaS-liaR
  Description: PubMed

  Regulation

  • liaG: constitutive

Biological materials

Mutant

• MGNA-B037 (yvqF::erm), available at the NBRP B. subtilis, Japan
• BKE33100 (ΔliaF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTGGTGTCCGCCTCC, downstream forward: _UP4_GGTGATGTGGATGTGAAGTA
• BKK33100 (ΔliaF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTGGTGTCCGCCTCC, downstream forward: _UP4_GGTGATGTGGATGTGAAGTA

References

Reviews

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Original publications

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