liaF [2019-06-06 09:44:59]

inhibitor of [protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|LiaS] kinase activity

locus: BSU33100

pI: 9.21

mw: 26.88 kDa

protein length: 241 aa

gene length: 726 bp

function: control of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR] activity

product: negative effector of [protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|LiaS]

essential: no

synonyms: yvqF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.3|Control of response regulators/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,396,114 → 3,396,839

The protein

Catalyzed reaction/ biological activity

• inhibitor of [protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|LiaS] kinase activity, maintains [protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|LiaS] in the phosphatase state in the absence of the stress signal [Pubmed|23279150]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
  description: [Pubmed|16816187]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15273097], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]: activation, [Pubmed|16816187], in [regulon|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR regulon]

  regulation

  • ''[protein|search|liaG]'': constitutive

• genes: [gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
  description: [Pubmed|14651641]

  regulation

  • ''[protein|search|liaG]'': constitutive

Biological materials

Mutant

• MGNA-B037 (yvqF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1036 NBRP B. subtilis, Japan]
• BKE33100 (Δ[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTGGTGTCCGCCTCC, downstream forward: _UP4_GGTGATGTGGATGTGAAGTA
• BKK33100 (Δ[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTGGTGTCCGCCTCC, downstream forward: _UP4_GGTGATGTGGATGTGAAGTA

References

Reviews

• 27344142

Original publications

• 19164152,20639339,15273097,17660417,16816187,15101989,17660417,16816187,23279150,20817675,22092710,23326432